In vitro regeneration and polyploidy induction in Pelargonium × hortorum L. H. Bailey

Abstract

The objective of this work was to induce in vitro shoot regeneration as influenced by plant growth re­gu­la­tors and ascertain an effective method of polyploidy induction using colchicine and oryzalin in two diploid cultivars ‘Gizela’ and ‘Black Velvet Scarlet F1’ of Pelargonium × hortorum L. H. Bailey. In both cultivars, benzyladenine (BA) significantly improved shoot regeneration compared to zeatin. The infiltration and the dip methods of explant treatment were used for polyploidy induction. Regenerants were analyzed using the flow cytometry (FCM). In ‘Gizela’ and ‘Black Velvet Scarlet F1’, 10 tetraploids on the level of 2n = 4x were found by the infiltration method. The tetraploidy was determined by the dip method in 4 shoots of ‘Gizela’ and 11 tetraploids were detected in ‘Black Velvet Scarlet F1’.

Keywords

• <i>Pelargonium</i> × <i>hortorum</i> L. H. Bailey cultivars
• <i>in vitro</i> shoot regeneration
• colchicine
• oryzalin
• polyploidization