Immunity response of mice infected with laser irradiated protoscoleces of Echinococcus granulosus

Abstract

The present study investigated the effect of the Neodymium- Doped Yttrium Aluminum Garnet (Nd: Y3AL5G12) Laser eradiation 1000 mw, against infection with secondary hydatid disease in BALB/c mice by inoculating the animals with protoscoleces of Echinococcus granulosus exposed to laser irradiation, for various periods 40,60,120 and 240 minutes, with viability of 70%,57%,50% and 40%, respectively, contrasted to the control set (mice inoculated with unexposed protoscoleces) along three months, depending on the evaluation of the total count of WBCs and assessment of acquired cell- mediated immunity, demonstrated by delayed type hypersensitivity test (DTH). The results showed considerable increase (p3, in comparison to the control group 5420 cell/cm3, after 120 minutes, two months post infection. Moreover, there was considerable excess (p Results revealed that irradiation of Echinococcus granulosus protoscoleces with laser had a significant influence on the production of WBCs and stimulation of delayed-type hypersensitivity reaction in mice.

Keywords

• hydatid disease
• echinococcus granulosus
• laser eradiation