Extraction of Nonstructural Carbon and Cellulose from Wood for Radiocarbon Analysis

Claudia Czimczik; Susan  Trumbore; Xiaomei  Xu; Mariah  Carbone; Andrew  Richardson

doi:10.21769/BioProtoc.1169

Bio-Protocol (Jul 2014)

Extraction of Nonstructural Carbon and Cellulose from Wood for Radiocarbon Analysis

Claudia Czimczik,
Susan Trumbore,
Xiaomei Xu,
Mariah Carbone,
Andrew Richardson

Affiliations

Claudia Czimczik: Earth System Science, University of California, Irvine, USA
Susan Trumbore: Biogeochemical Processes, Max Planck Institute for Biogeochemistry, Jena, Germany
Xiaomei Xu: Earth System Science, University of California, Irvine, USA
Mariah Carbone: Earth Systems Research Center, University of New Hampshire, Durham, NH, USA
Andrew Richardson: Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA

DOI: https://doi.org/10.21769/BioProtoc.1169
Journal volume & issue: Vol. 4, no. 13

Abstract

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This method aims at isolating nonstructural organic carbon (NSC) pools, i.e. soluble sugars and starch, from wood for radiocarbon (14C) analysis at natural abundance levels (≤1 ppt). Pools are operationally defined to 1) physically isolate pools - prohibiting the use of destructive methods, such as compound-specific enzyme digestion, and 2) minimize possible contamination with extraneous carbon form organic solvents.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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