Dolomite and Compost Amendments Enhance Cu Phytostabilization and Increase Microbiota of the Leachates from a Cu-Contaminated Soil
Laura Giagnoni,
Luiz Gustavo dos Anjos Borges,
Adriana Giongo,
Andressa de Oliveira Silveira,
Alexandria N. Ardissone,
Eric W. Triplett,
Michel Mench,
Giancarlo Renella
Affiliations
Laura Giagnoni
Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine 18, 50144 Florence, Italy
Luiz Gustavo dos Anjos Borges
Instituto do Petróleo e dos Recursos Naturais (IPR), Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681, Predio 96J, Porto Alegre 90619-900, Brazil
Adriana Giongo
Instituto do Petróleo e dos Recursos Naturais (IPR), Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681, Predio 96J, Porto Alegre 90619-900, Brazil
Andressa de Oliveira Silveira
Centro de Tecnologia, Departamento de Engenharia Sanitária e Ambiental, Universidade Federal de Santa Maria (UFSM), Santa Maria 97105-900, Brazil
Alexandria N. Ardissone
Institute of Food and Agricultural Sciences, Department of Microbiology and Cell Science, University of Florida (UF), Gainesville, FL 32611-0180, USA
Eric W. Triplett
Institute of Food and Agricultural Sciences, Department of Microbiology and Cell Science, University of Florida (UF), Gainesville, FL 32611-0180, USA
Michel Mench
University of Bordeaux, INRAE, BIOGECO (Biodiversity, Genes and Communities), F-33615 Pessac, France
Giancarlo Renella
Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padua, Viale dell’Università 16, 35020 Legnaro, Italy
The chemical properties, ecotoxicity, and microbiome of leachates from phytomanaged Cu-contaminated soils were analyzed. The phytomanagement was carried out using Cu-tolerant poplar Populus trichocarpa × deltoides cv. Beaupré and black bent Agrostis gigantea L., aided by soil amendments, i.e., dolomitic limestone (DL) and compost (OM), alone and in combination (OMDL). Plants plus either DL or OMDL amendments reduced in leachates the electrical conductivity, the Cu concentration, and the concentration of total organic C except for the OMDL treatment, and decreased leachate toxicity towards bacteria. Total N concentration increased in the OM leachates. The aided phytostabilization increased the culturable bacteria numbers and the proportion of Cu-resistant bacteria in the leachates, as compared to the leachate from the untreated soil. Phytomanagement also enriched the microbial communities of the leachates with plant beneficial bacteria. Overall, the Cu stabilization and phytomanagement induced positive changes in the microbial communities of the soil leachates.
