Perbandingan Hasil Pengukuran Intrusi Fluor pada Permukaan Email dengan Metoda Fluoresensi dan dengan Metoda Analisis Mikro EDX

Abstract

Fluoride intrusion into enamel surface is one of the important factors of success in topical fluoridation. Objective: to compare EDX and fluorescence measurement methods of fluoride intrusion into enamel surface after anchovy application. Methods: 5 extracted impacted third molars were immersed in sterile saline solution for 24 hours. The buccal surfaces of teeth were painted with nail varnish, and a window of 5 mm2 at the center of each surface was left unpainted. Dried anchovies from the market were heated and powdered, and 5 g of this anchovy powder was diluted in 100ml deionized distilled water to prepare an anchovy solution. The teeth were immersed in the anchovy solution for 5 min twice a day with 3 hours intervals. Immersions were repeated for 9 days. After immersion, the teeth were cut transversally through the window. The occlusal portions of the specimens were prepared for microscopic slides at ± 40 µm thickness. The cervical portions of the teeth were used as EDX specimens. Olympus BX41TF Fluorescence microscope was used to measure fluorescence bandwith. LEO scanning electron microscope with micro analyzer was used to measure fluoride intrusion. Increment steps of 5 µm from outer edge of the enamel to inner side were used as the points of EDX analysis. Paired t-test was used to analyze the intrusion results. Results: Fluoride intrusion depth measured using the fluorescence method was 11.49 ± 0.71 µm, while from the results of EDX analysis the average depth of fluoride intrusion was 20.24 ± 0.57 µm. Statistical analysis showed significant difference between the two methods. Conclusion: Intrusion measurement using EDX analysis gives higher fluoride intrusion than the fluorescence method.

Keywords

• Fluoride intrusion
• anchovy
• EDX and fluorescence