Modification of polyvinyl chloride membranes for mycotoxins detection

Abstract

In this work, we investigated modification and characterizations of modified-Polyvinyl Chloride (PVC) membranes for mycotoxin detections. The modification of PVC is a successfully nucleophilic substitution of Chloride atoms on the PVC membranes. The chemical compositions and characteristics of modified-PVC were characterized by Raman Microscope, NMR, GPC, DSC and FE-SEM. Sulfo-N-hydroxysuccinimide (Sulfo-NHS) and Carbodiimide EDC were used as an active ester functionalities with amino groups of modified-PVC membranes for covalent bonding of antibody. The antibody and mycotoxin interactions can be transduced directly by measuring the Differential Pulse Voltammetry (DPV). It was found that the zearalenone (ZEN) detections can be accomplished with these performances: sensitivity 14.1 ± 3 μA/μg, linear range 10−2 - 10−5 μg/ml and linearity 0.999.

Keywords

• Amino-PVC
• Immunosensor
• Zearalenone
• Differential pulse voltammetry