Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins

Abstract

Summary: The isolation of protein-RNA complexes in the “denaturing cross-linked RNA immunoprecipitation” (dCLIP) protocol is based on biotin-tagging proteins of interest, UV cross-linking RNA to protein in vivo, RNase protection assay, and isolating RNA-protein complexes under denaturing conditions over a streptavidin column. Insofar as conventional antibody-based CLIP assays have been challenging to apply to Polycomb complexes, dCLIP has been applied successfully and yields small RNA footprints from which de novo motif analysis can be performed to identify RNA binding motifs.For complete details on the use and execution of this protocol, please refer to Rosenberg et al. (2017).

Keywords

• Genetics
• Genomics
• Molecular Biology
• Molecular/Chemical Probes
• Protein Biochemistry
• Systems biology