In vitro Callus induction and Shoot proliferation in Jojoba Simmondsia chinesis (Link) Schn.

Abstract

From economic site;jojoba Simmondsia chinesis (Link) Schn. is very important shrub as its seeds contain high percent of oily wax . This wax composes natural and chemical charachteristics which makes shrub to be involved in many trade, manufacture and medical uses. In this study several interactions between auxins and cytokinins and gibbrellin were implemented to find out the best concentration for callus induction, vegetative growth and differentiation for experiments. BA at(2 or 5) mg/l, NAA at (0.018, 2, 2, 5) mg/l and GA3 at (0.1 or 0.2) mg/l were used. Results showed that highest percent for callus induction were (50 and 70) % respectively in the treatment (2) mg/l BA and (2) mg/l for both BA and NAA. The results revealed that using Kin. plus NAA with (0.1 or 0.2) mg/l GA3, was the best concentration lead to high percent of differentiation for vegetative shoot were (40 and 44) % respectively However, the length of vegetative shoots were (2 and 2.5) cm respectively. , this indicates the importance of the balance between these phytohormones required for shoot growth.