Genomic and Metabolomic Analyses of Streptomyces albulus with Enhanced ε-Poly-l-lysine Production Through Adaptive Laboratory Evolution

Xidong Ren; Xinjie Sun; Yan Chen; Xiangheng Xi; Yunzhe Ma; Xinyue Jiang; Xian Zhang; Chenying Wang; Deqiang Zhu; Xinli Liu

doi:10.3390/microorganisms13010149

Microorganisms (Jan 2025)

Genomic and Metabolomic Analyses of Streptomyces albulus with Enhanced ε-Poly-l-lysine Production Through Adaptive Laboratory Evolution

Xidong Ren,
Xinjie Sun,
Yan Chen,
Xiangheng Xi,
Yunzhe Ma,
Xinyue Jiang,
Xian Zhang,
Chenying Wang,
Deqiang Zhu,
Xinli Liu

Affiliations

Xidong Ren: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Xinjie Sun: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Yan Chen: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Xiangheng Xi: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Yunzhe Ma: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Xinyue Jiang: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Xian Zhang: Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
Chenying Wang: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Deqiang Zhu: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Xinli Liu: State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China

DOI: https://doi.org/10.3390/microorganisms13010149
Journal volume & issue: Vol. 13, no. 1
p. 149

Abstract

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ε-poly-l-lysine (ε-PL), a natural food preservative, has garnered widespread attention. It is mainly produced by Streptomyces albulus, but the production by wild-type strains fails to meet the demands of industrialization. To address this issue, adaptive laboratory evolution (ALE) was successfully employed in this study, subjecting S. albulus CICC 11022 to environmental stresses such as acidic pH and antibiotics (rifampicin, gentamicin, and streptomycin). As a result of ALE, an evolutionary strain S. albulus C214 was obtained, exhibiting an increase in ε-PL production and cell growth by 153.23% and 234.51%, respectively, as compared with the original strain. Genomic and metabolic analyses revealed that mutations occurred in genes responsible for transcriptional regulation, transporter, cell envelope, energy metabolism, and secondary metabolite synthesis, as well as the enrichment of metabolites involved in the biosynthesis of ε-PL. These findings hold great significance for elucidating the mechanism underlying ε-PL synthesis.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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