Stem Cell Research (Dec 2017)

Generation of induced pluripotent stem cells from a patient with Parkinson's disease carrying LRRK2 p.I2012T mutation

  • Chin-Hsien Lin,
  • Yu-Che Cheng,
  • Han-I Lin,
  • Ming-Ching Ho,
  • Yu-Hung Hsu,
  • Cheng-Hao Wen,
  • Hui-Wen Ko,
  • Huai-En Lu,
  • Ching-Ying Huang,
  • Patrick C.H. Hsieh

DOI
https://doi.org/10.1016/j.scr.2017.10.020
Journal volume & issue
Vol. 25, no. C
pp. 123 – 127

Abstract

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Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by interactions between genetic and environmental factors. Leucine rich repeat kinase (LRRK2) is the most prevalent mutation in autosomal-dominant inheritance of PD. Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with p.I2012T mutation in LRRK2 gene by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the 3 germ layers in vivo. This cellular model will provide a useful platform for further pathophysiological studies of PD.