Isolation of adult mouse microglia using their in vitro adherent properties

Zoe Woolf; Taylor J. Stevenson; Kevin Lee; Yewon Jung; Thomas I.H. Park; Maurice A. Curtis; Johanna M. Montgomery; Michael Dragunow

STAR Protocols (Jun 2021)

Isolation of adult mouse microglia using their in vitro adherent properties

Zoe Woolf,
Taylor J. Stevenson,
Kevin Lee,
Yewon Jung,
Thomas I.H. Park,
Maurice A. Curtis,
Johanna M. Montgomery,
Michael Dragunow

Affiliations

Zoe Woolf: Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Corresponding author
Taylor J. Stevenson: Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Department of Anatomy and Medical Imaging, The University of Auckland, Auckland, Private Bag 92019, New Zealand
Kevin Lee: Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Department of Physiology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand
Yewon Jung: Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Department of Physiology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand
Thomas I.H. Park: Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand
Maurice A. Curtis: Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Department of Anatomy and Medical Imaging, The University of Auckland, Auckland, Private Bag 92019, New Zealand
Johanna M. Montgomery: Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Department of Physiology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand
Michael Dragunow: Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Centre for Brain Research, The University of Auckland, Auckland, Private Bag 92019, New Zealand; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100518

Abstract

Read online

Summary: Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure microglia cultures from 4- to 14-month-old mouse brains using their adherent properties in vitro. These isolated microglia recapitulate the adherent properties of adult human microglia and present a more suitable model for studying age-related diseases.For complete details on the use and execution of this protocol in adult human microglia, please refer to Rustenhoven et al. (2016).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords