International Journal of COPD (Jul 2021)

Effects of Atmospheric Fine Particulate Matter and Its Carrier Microbes on Pulmonary Microecology in Patients with COPD

  • Che C,
  • Sun X,
  • Wu Y,
  • Ma L,
  • Hu Y,
  • Yang W,
  • Qi H,
  • Zhou Y

Journal volume & issue
Vol. Volume 16
pp. 2049 – 2063

Abstract

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Chunli Che, 1 Xiazhong Sun, 2 Yuhan Wu, 1 Lixin Ma, 2 Yueying Hu, 3 Weiyan Yang, 3 Hong Qi, 2 Yumin Zhou 4 1Harbin Medical University, Harbin, 150001, Heilongjiang, People’s Republic of China; 2State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China; 3Department of Respiratory Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, People’s Republic of China; 4State Key Laboratory of Respiratory Disease, National Center for Respiratory Diseases, Guangzhou Institute of Respiratory Diseases, The First Affiliated Hospital, Guangzhou Medical University, Guangdong, Guangdong Sheng, People’s Republic of ChinaCorrespondence: Hong Qi; Yumin Zhou Email [email protected]; [email protected]: The aim of this paper was to analyse the influence of atmospheric fine particulate matter (AFPM) and atmospheric microorganisms on the pulmonary microecology of chronic obstructive pulmonary disease (COPD) patients in northeast China.Methods: Collected bronchoalveolar lavage fluid (BALF) of COPD patients in the high-risk period (group A) and low-risk period (group B) of AFPM inhalation and samples of AFPM in the same time range (group C) were collected. DNA sample sequencing, the bacterial abundance, and diversity bioinformatics of BALFs were performed by methods of Illumina MiSeq™ platform and Mothur and Uclust.Results: A total of 58 samples were sequenced, including 22 samples from group A, 26 samples from group B and 10 samples from group C. A total of 2,005,790 bacterial sequences and 34,256 bacterial numbers were detected. Group B had the highest bacterial diversity of the three groups. Group B also had the highest bacterial abundance index value. There were differences in the classification of bacterial colonies for the three groups at the genus level. The types of bacteria in group C were more numerous than other groups, and group B was higher than group A, which indicates that there were more bacteria in BALF during the high-risk period of AFPM inhalation. The detection rates of Streptococcus, Mycoplasma, Roche, Pushia, Chlamydia trachomatis and Brucella for group C were significantly higher than group A. The COG and KEGG databases’ difference analysis results for the bacterial gene function abundance of group A and group B were 40.7% in group A and 38.9% in group B (R=0.098, P=0.006). The human disease abundance in group A and group B was 1.16% and 1.12%, respectively (P> 0.05).Conclusion: The increase in the concentration of AFPM can increase the diversity and abundance of bacteria in the BALF of stable COPD patients.Clinical Trial Registration Number: 2020XS04-02.Keywords: COPD, atmospheric fine particulate matter, bronchoalveolar lavage fluid, microbe, pulmonary microecology

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