Scientific Reports (Dec 2020)

Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta

  • Vishal Dinkar,
  • S. K. Jha,
  • Niharika Mallick,
  • M. Niranjana,
  • Priyanka Agarwal,
  • J. B. Sharma,
  • Vinod

DOI
https://doi.org/10.1038/s41598-020-78679-3
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 9

Abstract

Read online

Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs 276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs 276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs 276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.