Sensors (Jun 2018)

A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

  • Feng Liu,
  • Ailing Ding,
  • Jiushang Zheng,
  • Jiucun Chen,
  • Bin Wang

DOI
https://doi.org/10.3390/s18061769
Journal volume & issue
Vol. 18, no. 6
p. 1769

Abstract

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A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.

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