Emerging Microbes and Infections (Sep 2018)
N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro
- Minxi Wei,
- Daning Wang,
- Zhihai Li,
- Shuo Song,
- Xianglin Kong,
- Xiaobing Mo,
- Yurou Yang,
- Maozhou He,
- Zhongyi Li,
- Bo Huang,
- Zhijie Lin,
- Huirong Pan,
- Qingbing Zheng,
- Hai Yu,
- Ying Gu,
- Jun Zhang,
- Shaowei Li,
- Ningshao Xia
Affiliations
- Minxi Wei
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Daning Wang
- National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University
- Zhihai Li
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Shuo Song
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Xianglin Kong
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Xiaobing Mo
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Yurou Yang
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Maozhou He
- National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University
- Zhongyi Li
- Xiamen Innovax Biotech Company, Ltd
- Bo Huang
- Xiamen Innovax Biotech Company, Ltd
- Zhijie Lin
- Xiamen Innovax Biotech Company, Ltd
- Huirong Pan
- Xiamen Innovax Biotech Company, Ltd
- Qingbing Zheng
- National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University
- Hai Yu
- National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University
- Ying Gu
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Jun Zhang
- National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen University
- Shaowei Li
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- Ningshao Xia
- State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University
- DOI
- https://doi.org/10.1038/s41426-018-0158-2
- Journal volume & issue
-
Vol. 7,
no. 1
pp. 1 – 12
Abstract
Highlights • N-terminal truncations promote the soluble expression of HPV L1 proteins in E. coli and their self-assembly of T = 7 icosahedral particle in vitro • An HPV 9-valent vaccine candidate was formulated with E. coli-derived HPV 6, 11, 16, 18, 31, 33, 45, 52, and 58 VLPs, and conferred comparable immunogenicity with Gardasil 9
