Acta Societatis Botanicorum Poloniae (Jan 2015)
Heterogeneity of the acid phosphatase and ribonuclease from protocorms of the orchids Cymbidium Sw. and changes occurring after treatment with streptomycin
Abstract
The molecular forms of the acid phosphatase and RNase in protocorms of Cymbidium Sw. were studied by disc electrophoresis. The effect of streptomycin added to the culture medium on both enzymes was investigated. Significant changes in enzyme activity and electrophoretic patterns occured after addition of streptomycin at the beginning of culture growth. This indicates that the enzymes are affected by streptomycin in early stages of development of the protocorms.