Antigenic response of a live attenuated staphylococcus aureus vaccine in rabbits isolated from buffalo mastitic milk

Abstract

The study was undertaken to prepare and evaluate the antigenic response of experimental live attenuated staph. aureus vaccine isolated from buffalo mastitic milk in rabbits. A total of 30 rabbits were divided into 3 groups, A, B and C each having 10 animals. Group A was given single injection of vaccine, group B was vaccinated twice at two weeks interval while group C was kept as control. Serum samples were collected from 3 rabbits selected randomly from each group at weekly interval. From day 0 to 6 weeks post vaccination, antigenic response was evaluated trough anticoagulase, antihaemolysin and bacterial agglutination tests which showed that antigenic response was highly positive (P<0.05) at day 21 in group A and B while same was in group B at 28 day of post vaccination. The anticoagulase and antihaemolysin tests results were positive.