MATEC Web of Conferences (Jan 2017)

A Surface Plasmon Resonance Histamine Sensor Based on an Electropolymerized Molecularly Imprinted Polymer (E-MIP)

  • Hubilla Frances Adeline D.,
  • Mabilangan Arvin I.,
  • Advincula Rigoberto C.,
  • del Mundo Florian R.

DOI
https://doi.org/10.1051/matecconf/20179502006
Journal volume & issue
Vol. 95
p. 02006

Abstract

Read online

In this paper, histamine sensing by surface plasmon resonance (SPR) spectroscopy using an electropolymerized molecularly imprinted polymer (E-MIP) of functionalized polyterthiophene and histamine is reported. The MIP histamine sensor showed good linear relationship (R2 = 0.995) between the SPR angle shift and the logarithm of histamine concentration, ranging from 15 – 500 μg/mL, with a limit of detection (LOD) of 2.0 μg/mL and a calibration sensitivity of 44.6 m° per log μg/mL histamine. Results also showed higher binding affinity of histamine for the MIP sensor than for the non-imprinted (NIP) sensor indicating excellent specificity. Moreover, the selectivity of the MIP histamine sensor against some of its structural analogues was demonstrated.