Whole genome sequencing data and analyses of the underlying SUP35 transcriptional regulation for a Saccharomyces cerevisiae nonsense suppressor mutant
Andrew G. Matveenko,
Polina B. Drozdova,
Svetlana E. Moskalenko,
Oleg V. Tarasov,
Galina A. Zhouravleva
Affiliations
Andrew G. Matveenko
Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg 199034, Russia
Polina B. Drozdova
Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg 199034, Russia
Svetlana E. Moskalenko
Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg 199034, Russia; St. Petersburg Branch, Vavilov Institute of General Genetics, Russian Academy of Sciences, St. Petersburg 199034, Russia
Oleg V. Tarasov
Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg 199034, Russia
Galina A. Zhouravleva
Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg 199034, Russia; Laboratory of Amyloid Biology, Saint Petersburg State University, St. Petersburg 199034, Russia; Corresponding author at: Department of Genetics and Biotechnology, Saint-Petersburg State University, Universitetskaya emb., 7/9, St. Petersburg 199034, Russia.
Termination of translation in eukaryotes is governed by two release factors encoded by the SUP45 and SUP35 genes in Saccharomyces cerevisiae. Previously, a set of mutations in these genes had been obtained. However, the exact sequence change associated with one mutation, sup35-222, was not identified by Sanger sequencing of the SUP35 region. Presented here are whole-genome sequencing data for the sup35-222 strain, data on copy number variation in its genome along with supporting pulse-field gel electrophoresis experiment data, and the list of single-nucleotide variations that differentiate this strain and its wild-type ancestor. One substitution upstream the SUP35 gene was located in a sequence corresponding to the Abf1-binding site. Data obtained from the introduction of this variation from sup35-222 strain into a different wild-type strain, specifically, detection of a nonsense-suppressor phenotype accompanied by a decrease in the Sup35 protein level, are also presented in this article.
