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Performance of a Multiplex Serological Helicobacter pylori Assay on a Novel Microfluidic Assay Platform

Proteomes. 2017;5(4):24 DOI 10.3390/proteomes5040024

 

Journal Homepage

Journal Title: Proteomes

ISSN: 2227-7382 (Print)

Publisher: MDPI AG

LCC Subject Category: Science: Microbiology

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS


Angela Filomena (NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr 55, 72770 Reutlingen, Germany)

Anna Guenther (NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr 55, 72770 Reutlingen, Germany)

Hannes Planatscher (NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr 55, 72770 Reutlingen, Germany)

Francois Topin (MyCartis, Technologiepark 4, 9052 Zwijnaarde, Belgium)

Joseph She (MyCartis, Technologiepark 4, 9052 Zwijnaarde, Belgium)

Luca Formichella (Institut für medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Trogerstrasse 30, 81675 Munich, Germany)

Laurent Terradot (UMR 5086 Molecular Microbiology and Structural Biochemistry, CNRS-Université de Lyon 1, Institut de Biologie et Chimie des Protéines, 7 Passage du Vercors, 69367 Lyon Cedex 07, France)

Markus Gerhard (Institut für medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Trogerstrasse 30, 81675 Munich, Germany)

Thomas O. Joos (NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr 55, 72770 Reutlingen, Germany)

Hannelore Meyer (Institut für medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Trogerstrasse 30, 81675 Munich, Germany)

Nicole Schneiderhan-Marra (NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr 55, 72770 Reutlingen, Germany)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks

 

Abstract | Full Text

Infection with Helicobacter pylori (H. pylori) occurs in 50% of the world population, and is associated with the development of ulcer and gastric cancer. Serological diagnostic tests indicate an H. pylori infection by detecting antibodies directed against H. pylori proteins. In addition to line blots, multiplex assay platforms provide smart solutions for the simultaneous analysis of antibody responses towards several H. pylori proteins. We used seven H. pylori proteins (FliD, gGT, GroEL, HpaA, CagA, VacA, and HP0231) and an H. pylori lysate for the development of a multiplex serological assay on a novel microfluidic platform. The reaction limited binding regime in the microfluidic channels allows for a short incubation time of 35 min. The developed assay showed very high sensitivity (99%) and specificity (100%). Besides sensitivity and specificity, the technical validation (intra-assay CV = 3.7 ± 1.2% and inter-assay CV = 5.5 ± 1.2%) demonstrates that our assay is also a robust tool for the analysis of the H. pylori-specific antibody response. The integration of the virulence factors CagA and VacA allow for the assessment of the risk for gastric cancer development. The short assay time and the performance of the platform shows the potential for implementation of such assays in a clinical setting.