Evaluation of Biological Properties of New Selective Differential Medium for Cholera Vibrios Isolation Based on the Results of Laboratоry Trials

Abstract

Previously developed selective differential medium for V. cholerae growth was modernized. The modernized medium called SDMV-M was shown to possess the required sensitivity and germination index. The growth of E. coli was entirely inhibited, that of P. vulgaris was inhibited considerably. The medium possessed good differentiating ability: orange V. cholerae colonies were clearly distinguished from concomitant microorganisms. In the laboratory trials SDMV-M demonstrated some advantages as regards V. cholerae isolation from contaminated faeces in comparison with the reference medium TCBS.

Keywords

• cholera
• selective nutrient media
• selective medium for <i>v. cholerae</i> isolation
• laboratory trials