Genetic and Molecular Characterization of Maize Landraces from Central China

Abstract

Maize productivity in the central belt plays a significant role in the food security of China. With good adaptability and disease resistance, landrace germplasm is important for maize improvement. A total of 246 landrace accessions were collected from the maize belts in central China and genotyped with the SLAF-seq (Specific-Locus Amplified Fragment Sequencing) method, and 144,650 SNPs were obtained for each accession. The results showed that the landrace accessions could be divided into three major groups. In the cluster results, Group I included 64 accessions, which mainly belonged to the landrace of White horse teeth; Group II had 71 accessions, which mainly belonged to the lantern red landraces; the rest of the 116 accessions were clustered as Group III, including a variety of types landraces and seven indicator inbred lines. In the results of structure and multidimensional scaling, the accessions’ attribution differed with the clusters, the main reason for which is the attribution change in intermediate germplasms. Linkage disequilibrium decay distance was 0.98 kb, which was much lower than that of temperate and tropical maize inbred lines, indicated the much higher genetic diversity of landrace germplasms. The results can help us select suitable landrace germplasms and speed up the process of inbred line development and maize improvement.

Keywords

• maize
• landrace
• genetic diversity
• cluster analysis
• heterotic groups