Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation

Tong Xu; Junyu Chen; Ruchira Mitra; Lin Lin; Zhengwei Xie; Guo-Qiang Chen; Hua Xiang; Jing Han

doi:10.1038/s42003-022-03570-y

Communications Biology (Jun 2022)

Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation

Tong Xu,
Junyu Chen,
Ruchira Mitra,
Lin Lin,
Zhengwei Xie,
Guo-Qiang Chen,
Hua Xiang,
Jing Han

Affiliations

Tong Xu: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Junyu Chen: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Ruchira Mitra: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Lin Lin: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Zhengwei Xie: Peking University International Cancer Institute, Health Science Center, Peking University
Guo-Qiang Chen: Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
Hua Xiang: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
Jing Han: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s42003-022-03570-y
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 11

Abstract

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CRISPR/Cas9 editing of a PHB-producing H. bluephagenesis strain is used to delete the redundant synthesis pathways of flagella and EPSs, allowing for enhanced self-flocculation, less carbon and energy requirement for metabolic processes and feasible electrotransformation.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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