Effect of local application of leukocyte and platelet-rich fibrin (L-PRF) on postoperative swelling and salivary IL-6 levels after surgical removal of impacted mandibular third molars

Abstract

Aims: To measure the effects of leukocyte and platelet-rich fibrin (L-PRF) on swelling and saliva levels of interleukin-6 (IL-6) at different intervals after surgical removal of the impacted lower third molar. Materials and Methods: The study included 30 patients aged between (17-35) years who needed surgical removal of an impacted lower third molar under local anesthesia. Patients were divided into two groups, L-PRF, and control groups. In the L-PRF group, the L-PRF was prepared from the patient’s own blood, and the blood was centrifuged for 12 minutes at 2700 rpm. The clot was inserted in the socket after surgical removal of the impacted lower wisdom teeth, while in the control group, no material was inserted inside the socket. Facial swelling was assessed preoperatively, 2nd, and 7th days after the operation. Saliva level of IL-6 was measured (using ELISA) preoperatively and 7th day after the operation. Results: Regarding swelling within groups, in the L-PRF group, swelling showed a significant difference, while in the control group, there was no statistically significance difference. Between groups, there was no statistical difference in swelling between the preoperative and 2nd day postoperatively, and between 2nd and 7th day after the operation. Regarding IL-6 levels on the 7th postoperative day, there were no statistically significant differences between both groups. Conclusions: L-PRF decreases swelling following surgery within the group, but differences in swelling and levels of IL-6 in saliva among groups were not statistically significant, after surgical removal of the impacted mandibular third molar.

Keywords

• l-prf
• il-6 in saliva
• impacted mandibular third molar
• swelling