Molecules (May 2021)

Droplet Microfluidic Optimisation Using Micropipette Characterisation of Bio-Instructive Polymeric Surfactants

  • Charlotte A. Henshaw,
  • Adam A. Dundas,
  • Valentina Cuzzucoli Crucitti,
  • Morgan R. Alexander,
  • Ricky Wildman,
  • Felicity R. A. J. Rose,
  • Derek J. Irvine,
  • Philip M. Williams

DOI
https://doi.org/10.3390/molecules26113302
Journal volume & issue
Vol. 26, no. 11
p. 3302

Abstract

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Droplet microfluidics can produce highly tailored microparticles whilst retaining monodispersity. However, these systems often require lengthy optimisation, commonly based on a trial-and-error approach, particularly when using bio-instructive, polymeric surfactants. Here, micropipette manipulation methods were used to optimise the concentration of bespoke polymeric surfactants to produce biodegradable (poly(d,l-lactic acid) (PDLLA)) microparticles with unique, bio-instructive surface chemistries. The effect of these three-dimensional surfactants on the interfacial tension of the system was analysed. It was determined that to provide adequate stabilisation, a low level (0.1% (w/v)) of poly(vinyl acetate-co-alcohol) (PVA) was required. Optimisation of the PVA concentration was informed by micropipette manipulation. As a result, successful, monodisperse particles were produced that maintained the desired bio-instructive surface chemistry.

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