EMBO Molecular Medicine (Sep 2022)
A catalog of numerical centrosome defects in epithelial ovarian cancers
- Jean‐Philippe Morretton,
- Anthony Simon,
- Aurélie Herbette,
- Jorge Barbazan,
- Carlos Pérez‐González,
- Camille Cosson,
- Bassirou Mboup,
- Aurélien Latouche,
- Tatiana Popova,
- Yann Kieffer,
- Anne‐Sophie Macé,
- Pierre Gestraud,
- Guillaume Bataillon,
- Véronique Becette,
- Didier Meseure,
- André Nicolas,
- Odette Mariani,
- Anne Vincent‐Salomon,
- Marc‐Henri Stern,
- Fatima Mechta‐Grigoriou,
- Sergio Roman Roman,
- Danijela Matic Vignjevic,
- Roman Rouzier,
- Xavier Sastre‐Garau,
- Oumou Goundiam,
- Renata Basto
Affiliations
- Jean‐Philippe Morretton
- Biology of Centrosomes and Genetic Instability, Institut Curie PSL Research University, CNRS UMR 144 Paris France
- Anthony Simon
- Biology of Centrosomes and Genetic Instability, Institut Curie PSL Research University, CNRS UMR 144 Paris France
- Aurélie Herbette
- Department of Translational Research, Institut Curie PSL University Paris Cedex 05 France
- Jorge Barbazan
- Migration and Invasion Laboratory, Institut Curie PSL Research University, CNRS UMR 144 Paris France
- Carlos Pérez‐González
- Migration and Invasion Laboratory, Institut Curie PSL Research University, CNRS UMR 144 Paris France
- Camille Cosson
- Department of Translational Research, Institut Curie PSL University Paris Cedex 05 France
- Bassirou Mboup
- Statistical Methods for Precision Medicine INSERM U900, Institut Curie Saint‐Cloud France
- Aurélien Latouche
- Statistical Methods for Precision Medicine INSERM U900, Institut Curie Saint‐Cloud France
- Tatiana Popova
- DNA Repair & Uveal Melanoma (D.R.U.M.), INSERM U830, Institut Curie PSL Research University Paris Cedex 05 France
- Yann Kieffer
- Stress and Cancer Laboratory, INSERM U830, Institut Curie, Team Ligue Nationale Contre le Cancer PSL Research University Paris France
- Anne‐Sophie Macé
- Cell and Tissue Imaging Facility (PICT‐IBiSA), Institut Curie PSL Research University, Centre National de la Recherche Scientifique Paris France
- Pierre Gestraud
- Bioinformatics and Computational Systems Biology of Cancer, Mines Paristech, INSERM U900, Institut Curie PSL University Paris Cedex 05 France
- Guillaume Bataillon
- Department of Pathology Institut Curie Paris Cedex 05 France
- Véronique Becette
- Department of Pathology Institut Curie Paris Cedex 05 France
- Didier Meseure
- Department of Pathology Institut Curie Paris Cedex 05 France
- André Nicolas
- Department of Pathology Institut Curie Paris Cedex 05 France
- Odette Mariani
- Department of Pathology Institut Curie Paris Cedex 05 France
- Anne Vincent‐Salomon
- Department of Pathology Institut Curie Paris Cedex 05 France
- Marc‐Henri Stern
- DNA Repair & Uveal Melanoma (D.R.U.M.), INSERM U830, Institut Curie PSL Research University Paris Cedex 05 France
- Fatima Mechta‐Grigoriou
- Stress and Cancer Laboratory, INSERM U830, Institut Curie, Team Ligue Nationale Contre le Cancer PSL Research University Paris France
- Sergio Roman Roman
- Department of Translational Research, Institut Curie PSL University Paris Cedex 05 France
- Danijela Matic Vignjevic
- Migration and Invasion Laboratory, Institut Curie PSL Research University, CNRS UMR 144 Paris France
- Roman Rouzier
- Statistical Methods for Precision Medicine INSERM U900, Institut Curie Saint‐Cloud France
- Xavier Sastre‐Garau
- Department of Pathology Institut Curie Paris Cedex 05 France
- Oumou Goundiam
- Department of Translational Research, Institut Curie PSL University Paris Cedex 05 France
- Renata Basto
- Biology of Centrosomes and Genetic Instability, Institut Curie PSL Research University, CNRS UMR 144 Paris France
- DOI
- https://doi.org/10.15252/emmm.202215670
- Journal volume & issue
-
Vol. 14,
no. 9
pp. n/a – n/a
Abstract
Abstract Centrosome amplification, the presence of more than two centrosomes in a cell is a common feature of most human cancer cell lines. However, little is known about centrosome numbers in human cancers and whether amplification or other numerical aberrations are frequently present. To address this question, we have analyzed a large cohort of primary human epithelial ovarian cancers (EOCs) from 100 patients. We found that rigorous quantitation of centrosome number in tumor samples was extremely challenging due to tumor heterogeneity and extensive tissue disorganization. Interestingly, even if centrosome clusters could be identified, the incidence of centrosome amplification was not comparable to what has been described in cultured cancer cells. Surprisingly, centrosome loss events where a few or many nuclei were not associated with centrosomes were clearly noticed and overall more frequent than centrosome amplification. Our findings highlight the difficulty of characterizing centrosome numbers in human tumors, while revealing a novel paradigm of centrosome number defects in EOCs.
Keywords
