Activity of Hepatic Enzymes of Isolated Hepatocytes under the Influence of Copper Acetate

Abstract

The article describes a biochemical method for characterizing the hepatocyte culture under the action of CA. Isolated primary hepatocyte culture was exposed to CA in different concentrations, and then the activity of ALT, AST, ALP, and the amount of glucose, in the culture medium was determined. The result showed that the level of cell membrane damage and the release of ALT and AST into the extracellular space depend on the concentration of the acting substance, and, in addition, AST was found to be more sensitive to the toxic effects of CA. However, the activity of ALP proved to be an indicator of the copper ions' action on the enzyme but not of destructive processes in the hepatocyte culture. At the same time, a decrease in the glucose level may be used as a characteristic of the cell metabolic activity. Based on the results obtained, we can conclude that proposed analysis methods allow us to comprehensively characterize the processes occurring with cultured hepatocytes under the influence of CA.

Keywords

• hepatocytes
• alanine aminotransferase
• aspartate aminotransferase
• alkaline phosphatase