Cement glands as a second sexual characteristic can help to evaluate the ovarian development and reproductive spawning period of the female crayfish Procambarus clarkii

Jinghao Li; Chen Qian; Wenzong Zhou; Yewen Xi; Yongxu Cheng; Jiayao Li

Aquaculture Reports (Apr 2024)

Cement glands as a second sexual characteristic can help to evaluate the ovarian development and reproductive spawning period of the female crayfish Procambarus clarkii

Jinghao Li,
Chen Qian,
Wenzong Zhou,
Yewen Xi,
Yongxu Cheng,
Jiayao Li

Affiliations

Jinghao Li: Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Chen Qian: Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Wenzong Zhou: Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
Yewen Xi: Anhui fisheries Technology Extension Center, Anhui, 230001, China
Yongxu Cheng: Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Corresponding authors at: Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
Jiayao Li: Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Corresponding authors at: Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.

Journal volume & issue: Vol. 35
p. 101977

Abstract

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Procambarus clarkii is an important economic species in freshwater culture. The secondary sexual characteristics of male crayfish have been studied, but little is known about female crayfish. In this study, it is found that cement glands appeared in the abdominal pleopods and telson of female crayfish during spawning period. The relationship between the appearance of cement glands and the ovarian development, mating behavior and spawning was studied, the histological change, proteome profiles of cement glands before and spawning were compared. The results are as follows: Cement glands was also a secondary characteristic of female crayfish. It had no significant promotion on mating behavior. Compared to crayfish without cement glands, female crayfish with cement glands had better developed ovary, manifested in higher gonadal index and oocyte diameter and significant lower VgR gene expression. The cement glands disappeared after the female crayfish spawned. The results of proteome profiles showed that about 312 proteins were identified in cement glands. These proteins possibly participate in maintaining the osmotic pressure of sperm and eggs, protecting and promoting the combination of fertilized eggs, and promoting the adhesion of eggs to the abdomen pleopods during the spawning period. Based on the characteristic of cement glands, it is inferred that the function of cement glands is mainly to prepare for spawning and egg hatching of female crayfish. The cement glands can be used as an indicator to help to evaluate the ovarian development and spawning period of female P.clarkii.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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