Pharmaceutical Sciences and Research (Apr 2015)

Aktivitas Antioksidan Ekstrak, Fraksi dan Golongan Senyawa Kimia Daun Premna oblongata Miq.

  • Katrin,
  • Atika Bendra

DOI
https://doi.org/10.7454/psr.v2i1.3332
Journal volume & issue
Vol. 2, no. 1
pp. 21 – 31

Abstract

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Indonesia has many kinds of plants which can be used as natural antioxidant sources. This study was focused on antioxidant activity of Cincau Perdu leaves extract (Premna oblongata Miq.) using 1-Diphenyl-2-picrylhydrazyl (DPPH) methods. P. oblongata leaves was extracted using n-hexane, ethylacetate and methanol. It was found that methanol extract has the highest activity with IC50 value of 20.01 μg/mL. The extract with highest antioxidant activity were fractionated with accelerated column chromatography and were earned 6 combination fractions. The antioxidant activity of each combination fractions were tested by DPPH methods. Fraction-5 showed the highest activity as antioxidant with IC50 of 23.51 μg/mL. The compounds of the active fractions were flavonoid, glikon, saponin and tannin.

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