ANTI-INFLAMMATORY ACTIVITY OF ALSTONIA SCHOLARIS IN ALBINO RATS

Abstract

Evaluation of anti-inflammatory activities along with the phytochemical screening of hot methanolic extract of A. scholaris stem bark (ASE) in albino rats was undertaken. The preliminary phytochemical screening of the plant revealed the presence of tannins, alkaloids, saponins, phystosterols, phenolic compounds, glycoside and flavonoids. Oral LD50 of ASE by limit test was found to be above 2000 mg/kg. Two dose level of 200 (1/10 LD50) and 400 mg/kg (1/5 LD50) were selected for studying the anti -inflammatory activity of ASE using the carrageenan - induced acute paw oedema model in rats. The extract showed significant (p < 0.01) dose dependent reduction in rat paw oedema. The percentages of inhibition of oedema were 42.55 and 53.19 with 200 and 400 mg/kg, p.o. doses of ASE, respectively, as compared to control. The anti-inflammatory action of ASE can be attributed to its flavonoid contents, which are known to act through inhibition of prostaglandin biosynthesis.

Keywords

• A. scholaris
• Albino rats
• Oral LD50
• Acute paw oedema
• Anti-inflammatory
• Flavonoid