Electrospinning Live Cells Using Gelatin and Pullulan

Nasim Nosoudi; Anson  Jacob Oommen; Savannah Stultz; Micah Jordan; Seba Aldabel; Chandra Hohne; James Mosser; Bailey Archacki; Alliah Turner; Paul Turner

doi:10.3390/bioengineering7010021

Bioengineering (Feb 2020)

Electrospinning Live Cells Using Gelatin and Pullulan

Nasim Nosoudi,
Anson Jacob Oommen,
Savannah Stultz,
Micah Jordan,
Seba Aldabel,
Chandra Hohne,
James Mosser,
Bailey Archacki,
Alliah Turner,
Paul Turner

Affiliations

Nasim Nosoudi: Department of biomedical engineering, College of Engineering and Computer Sciences (CECS), Marshall University, Weisberg Family Applied Engineering Complex, Huntington, WV 25755, USA
Anson Jacob Oommen: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Savannah Stultz: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Micah Jordan: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Seba Aldabel: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Chandra Hohne: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
James Mosser: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Bailey Archacki: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Alliah Turner: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA
Paul Turner: Wright State University, Biomedical, Industrial and Human Factors Engineering, 228 Russ Engineering, 3640 Colonel Glenn Hwy, Dayton, OH 45435, USA

DOI: https://doi.org/10.3390/bioengineering7010021
Journal volume & issue: Vol. 7, no. 1
p. 21

Abstract

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Electrospinning is a scaffold production method that utilizes electric force to draw a polymer solution into nanometer-sized fibers. By optimizing the polymer and electrospinning parameters, a scaffold is created with the desired thickness, alignment, and pore size. Traditionally, cells and biological constitutes are implanted into the matrix of the three-dimensional scaffold following electrospinning. Our design simultaneously introduces cells into the scaffold during the electrospinning process at 8 kV. In this study, we achieved 90% viability of adipose tissue-derived stem cells through electrospinning.

Published in Bioengineering

ISSN: 2306-5354 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology; Science: Biology (General)
Website: https://www.mdpi.com/journal/bioengineering

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