Data set for transcriptional response to depletion of the Shoc2 scaffolding protein
Eric C. Rouchka,
Myoungkun Jeoung,
Eun Ryoung Jang,
Jinpeng Liu,
Chi Wang,
Xiaohong Li,
Emilia Galperin
Affiliations
Eric C. Rouchka
Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40292, United States; Kentucky Biomedical Research Infrastructure Network Bioinformatics Core, University of Louisville, Louisville, KY 40292, United States
Myoungkun Jeoung
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, United States
Eun Ryoung Jang
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, United States
Jinpeng Liu
Markey Cancer Center and Division of Biostatistics, University of Kentucky, Lexington, KY 40536, United States
Chi Wang
Markey Cancer Center and Division of Biostatistics, University of Kentucky, Lexington, KY 40536, United States
Xiaohong Li
Kentucky Biomedical Research Infrastructure Network Bioinformatics Core, University of Louisville, Louisville, KY 40292, United States; Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, United States; Department of Bioinformatics and Biostatistics, University of Louisville, Louisville, KY 40292, United States
Emilia Galperin
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, United States; Corresponding author.
The Suppressor of Clear, Caenorhabditis elegans Homolog (SHOC2) is a scaffold protein that positively modulates activity of the RAS/ERK1/2 MAP kinase signaling cascade. We set out to understand the ERK1/2 pathway transcriptional response transduced through the SHOC2 scaffolding module. This data article describes raw gene expression within triplicates of kidney fibroblast-like Cos1 cell line expressing non-targeting shRNA (Cos-NT) and triplicates of Cos1 cells depleted of SHOC2 using shRNA (Cos-LV1) upon activation of ERK1/2 pathway by the Epidermal Growth Factor Receptor (EGFR). The data referred here is available in NCBI׳s Gene Expression Omnibus (GEO), accession GEO: GSE67063 as well as NCBI׳s Sequence Read Archive (SRA), accession SRA: SRP056324. A complete analysis of the results can be found in “Shoc2-tranduced ERK1/2 motility signals – Novel insights from functional genomics”(Jeoung et al., 2016) [1].
