Identification of bioactive compounds in leaf extract of Avicennia alba by GC-MS analysis and evaluation of its in-vitro anticancer potential against MCF7 and HeLa cell lines

Abstract

The current study focused on GC-MS analysis for identification of bioactive compounds in leaf extract of mangrove plant, Avicennia alba and the leaf extract of Avicennia alba was tested for cytotoxicity against MCF 7 and HeLa cell lines. The spectral properties (GC-MS) of each separated compound were determined and found the different compounds namely terpenoids, di-terpene alcohols, tri-terpenes and phenolic compounds. GC-MS analysis proved that the leaf extract of Avicennia alba contain a high content of phenolic compounds. The methanol extract showed potent cytotoxicity against Human breastadeno carcinoma (MCF7) and HeLa cell lines, the viability of cancerous cells is reduced to 44.68% for MCF 7 and 35.89% for HeLa cells. The high anticancer activity was found against HeLa cell lines than MCF 7 cell lines. Keywords: Anticancer, HeLa cells, MCF 7 cells, MTT assay, GC-MS