<i>Salvia connivens</i> Methanolic Extract Against <i>Spodoptera frugiperda</i> and <i>Tenebrio molitor</i> and Its Effect on <i>Poecilia reticulata</i> and <i>Danio rerio</i>

Abstract

Spodoptera frugiperda (Lepidoptera: Noctuidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) are two prominent pests of maize and its stored grains, respectively. Botanical pesticides have been proposed as an alternative for their management. This study evaluated the insecticidal activity of Salvia connivens (Lamiaceae) methanolic extract and rosmarinic acid against S. frugiperda and T. molitor by adding them to an artificial diet, as well as their ecotoxicological effects on Poecilia reticulata (Cyprinodontiformes: Poeciliidae) and Danio rerio (Cypriniformes: Danionidae) through acute toxicity tests. The methanolic extract showed higher mortality activity against S. frugiperda (LC50 = 874.28 ppm) than against T. molitor (LC50 = 1856.94 ppm) and was non-toxic to fish. Rosmarinic acid, the most abundant compound in the extract (80.45 mg g−1), showed higher activity against S. frugiperda (LC50 = 176.81 ppm). This compound did not cause a toxic effect on adult P. reticulata at the tested concentrations. However, in P. reticulata fingerlings and D. rerio adults, it was non-toxic, except in D. rerio embryos, where it was slightly toxic. These findings suggest that S. connivens methanolic extract has potential as a botanical product for the management of S. frugiperda and T. molitor with low ecotoxicological impact, while rosmarinic acid may be a useful compound for the management of S. frugiperda.

Keywords

• zebra fish
• guppy
• fall armyworm
• yellow mealworm
• rosmarinic acid