Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry for target determination of bioactive compounds in Dendrobium spp.
Jingjing Liang,
Ruyan Chen,
Wenping Zhang,
Lei Luo,
Yun Wang,
Qing Shen
Affiliations
Jingjing Liang
Zhejiang Provincial Institute for Food and Drug Control, Key Laboratory of Quality and Safety of Functional Food for State Market Regulation, Zhejiang Provincial Key Laboratory of Quality and Safety of Health Food for Market Supervision, Hangzhou, 310052, China
Ruyan Chen
Jiande Center for Disease Prevention and Control, Jiande, 311600, China
Wenping Zhang
Zhejiang Provincial Institute for Food and Drug Control, Key Laboratory of Quality and Safety of Functional Food for State Market Regulation, Zhejiang Provincial Key Laboratory of Quality and Safety of Health Food for Market Supervision, Hangzhou, 310052, China
Lei Luo
Zhejiang Provincial Institute for Food and Drug Control, Key Laboratory of Quality and Safety of Functional Food for State Market Regulation, Zhejiang Provincial Key Laboratory of Quality and Safety of Health Food for Market Supervision, Hangzhou, 310052, China
Yun Wang
Zhejiang Provincial Institute for Food and Drug Control, Key Laboratory of Quality and Safety of Functional Food for State Market Regulation, Zhejiang Provincial Key Laboratory of Quality and Safety of Health Food for Market Supervision, Hangzhou, 310052, China
Qing Shen
Panvascular Diseases Research Center, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, 324000, China; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, 310018, China; Corresponding author. Panvascular Diseases Research Center, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, 324000, China.
As a medicine-food homology herb, Dendrobium spp. has versatile applications in modern medicine and food industry. Herein, an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) based method was established for simultaneous quantification of six active components, including gigantol, erianin, naringenin, quercetin, rutin, and p-coumaric acid in Dendrobium spp., on the basis of optimized sample preparation, mass spectrometry conditions, and chromatography conditions. Sample extraction was carried out using methanol at a temperature of 60 °C, followed by separation on a T3 C18 column utilizing a gradient eluting program. The results demonstrated excellent linearity (r > 0.999) for the six active components within a specified concentration range. The average recovery rates ranged from 84.7 % to 106.9 %, and the precision (RSD) was within 7.4 %. The detection and quantification limits of this method ranged from 0.34 to 4.17 ng mL−1 and 1.12–13.91 ng mL−1, respectively. The established method demonstrates high accuracy and reliability and is applicable in practical sample detection. Different Dendrobium spp. exhibit specific variations in compound composition, with D. fimbriatum Hook. having a higher content of benzyl compounds and D. crystallinum. Rchb. f. having a higher content of flavonoids. This study provides experimental evidence for the quality and safety regulation of Dendrobium spp.
