Biofabrication of silver nanoparticles from Pseudomonas fluorescens to control tobacco mosaic virus

Abstract

Abstract A microbe-based silver nanoparticle could inhibit the tobacco mosaic virus. In the present study, AgNO3 reacts with Pseudomonas fluorescens CZ strain fermentative broth and formed silver nanoparticles. The crystallinity and purity of silver nanoparticles (AgNPs) were examined by an X-ray diffractometer. Number of Bragg reflection was indexed in the face-centered AgNPs to show a cubic structure. Observation of scan electron microscope (SEM) confirmed the formation of AgNPs. Antiviral effects determined by percentage inhibition of local lesion of tobacco mosaic virus (TMV). Spray of AgNPs and inoculation with TMV assayed analysis revealed percentage inhibition of local lesion 92.70. Effects of AgNPs on the morphology of TMV were observed by a transmission electron microscope (TEM). Micrograph images confirmed the significant effects on the morphology of TMV. Biogenic NP from AgNO3 and fermented broth from Pseudomonas fluorescens could be a potent approach to control the TMV.

Keywords

• Silver nanoparticles
• Tobacco mosaic virus
• X-ray diffractometer
• Transmission electron microscope
• Pseudomonas fluorescens