Time-Restricted Feeding Shifts the Skin Circadian Clock and Alters UVB-Induced DNA Damage
Hong Wang,
Elyse van Spyk,
Qiang Liu,
Mikhail Geyfman,
Michael L. Salmans,
Vivek Kumar,
Alexander Ihler,
Ning Li,
Joseph S. Takahashi,
Bogi Andersen
Affiliations
Hong Wang
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China; Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA; Department of Cell Biology and Genetics, School of Preclinical Medicine, Guangxi Medical University, Nanning 530021, China
Elyse van Spyk
Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA
Qiang Liu
Department of Computer Science, University of California, Irvine, Irvine, CA 92697, USA
Mikhail Geyfman
Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA
Michael L. Salmans
Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA
Vivek Kumar
The Jackson Laboratory, Bar Harbor, ME 04609, USA
Alexander Ihler
Department of Computer Science, University of California, Irvine, Irvine, CA 92697, USA
Ning Li
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
Joseph S. Takahashi
Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Bogi Andersen
Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA; Department of Medicine, Division of Endocrinology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Center for Complex Biological Systems, University of California, Irvine, Irvine, CA 92697, USA; Institute for Genomics and Bioinformatics, University of California, Irvine, Irvine, CA 92697, USA; Corresponding author
Summary: The epidermis is a highly regenerative barrier protecting organisms from environmental insults, including UV radiation, the main cause of skin cancer and skin aging. Here, we show that time-restricted feeding (RF) shifts the phase and alters the amplitude of the skin circadian clock and affects the expression of approximately 10% of the skin transcriptome. Furthermore, a large number of skin-expressed genes are acutely regulated by food intake. Although the circadian clock is required for daily rhythms in DNA synthesis in epidermal progenitor cells, RF-induced shifts in clock phase do not alter the phase of DNA synthesis. However, RF alters both diurnal sensitivity to UVB-induced DNA damage and expression of the key DNA repair gene, Xpa. Together, our findings indicate regulation of skin function by time of feeding and emphasize a link between circadian rhythm, food intake, and skin health. : Little is known about the effect of time of feeding on skin function. Wang et al. find that time-restricted feeding schedules affect skin gene expression, epidermal progenitor cell proliferation, and UVB-induced DNA damage, pointing to a modulatory role for food-intake timing in skin biology. Keywords: skin, circadian clock, time-restricted feeding, cell cycle, metabolism, DNA damage, aging
