Novel Micrococcus unila to Produce Glucosamine by Solid-state Fermentation of Shrimp Shell Waste

Wawan Abdullah  Setiawan; Andi Setiawan; Nafila Khansa  Salsabila; Widyastuti  Widyastuti; Aspita  Laila; Ni Luh Gede Ratna  Juliasih; Bambang  Irawan; Peni  Ahmadi; Ety  Apriliana; Masayoshi  Arai; John Hendri

doi:10.26554/sti.2024.9.4.779-789

Science and Technology Indonesia (Oct 2024)

Novel Micrococcus unila to Produce Glucosamine by Solid-state Fermentation of Shrimp Shell Waste

Wawan Abdullah Setiawan,
Andi Setiawan,
Nafila Khansa Salsabila,
Widyastuti Widyastuti,
Aspita Laila,
Ni Luh Gede Ratna Juliasih,
Bambang Irawan,
Peni Ahmadi,
Ety Apriliana,
Masayoshi Arai,
John Hendri

Affiliations

Wawan Abdullah Setiawan: Students of Doctoral Program, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Andi Setiawan: Department of Chemistry, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Nafila Khansa Salsabila: Research Assistant, Department of Chemistry, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Widyastuti Widyastuti: Department of Chemistry, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Aspita Laila: Department of Chemistry, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Ni Luh Gede Ratna Juliasih: Department of Chemistry, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Bambang Irawan: Department of Biology, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia
Peni Ahmadi: Research Center for Vaccine and Drug, Research Organization for Health, National Research and Innovation (BRIN), Cibinong, 16911, Indonesia
Ety Apriliana: Faculty of Medicine, Lampung Univeristy, Bandar Lampung, 35145, Indonesia
Masayoshi Arai: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka, 565-0871, Japan
John Hendri: Department of Chemistry, Faculty of Mathemathics and Natural Science, Lampung University, Bandar Lampung, 35145, Indonesia

DOI: https://doi.org/10.26554/sti.2024.9.4.779-789
Journal volume & issue: Vol. 9, no. 4
pp. 779 – 789

Abstract

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This study aimed to assess glucosamine production through enzymatic activity, utilizing actinomycetes sourced from shrimp shell waste (SSW) in a solid-state fermentation (SSF) process. A total of 16 actinomycetes underwent chitinase activity screening, and the strain exhibiting the highest chitinolytic index was chosen for subsequent morphological and phylogenetic analyses. High Performance Liquid Chromatography (HPLC) was employed to analyze glucosamine produced from the bioconversion of SSW via SSF. Optimal conditions for glucosamine production were determined by varying time, pH, and temperature. Isolate 18D36-A2 showed the highest chitinolytic index of 1.02 in the 32-mm clean zone. Phylogenetic analysis revealed 97% similarity to the genus Micrococcus, identifying it as a novel Micrococcus unila strain 18D36-A2 and deposited in GenBank. This isolate effectively converted shrimp shells. The findings showcase the bioconversion of SSW to glucosamine through SSF using the Micrococcus unila 18D36-A2. Furthermore, this study establishes a foundation for future research on environmentally friendly and sustainable designs for glucosamine production.

Published in Science and Technology Indonesia

ISSN: 2580-4405 (Print); 2580-4391 (Online)
Publisher: Magister Program of Material Sciences, Graduate School of Universitas Sriwijaya
Country of publisher: Indonesia
LCC subjects: Technology; Science
Website: https://sciencetechindonesia.com/index.php/jsti

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