Marine Drugs (Oct 2013)

Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae)

  • Cláudia Maria Oliveira Simões,
  • Eloir Paulo Schenkel,
  • Flávio Henrique Reginatto,
  • Jorge Alejandro Palermo,
  • Gabriela Cabrera,
  • João Luís Carraro,
  • Maria Izabel Goulart Moritz,
  • Éverson Miguel Bianco,
  • Maria Tereza Rojo de Almeida,
  • Simone Quintana de Oliveira,
  • Caroline Rigotto Borges,
  • Tatiana da Rosa Guimarães,
  • Carlos Guillermo Quiroz

DOI
https://doi.org/10.3390/md11114176
Journal volume & issue
Vol. 11, no. 11
pp. 4176 – 4192

Abstract

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The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.

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