Nitrated Fatty-Acids Distribution in Storage Biomolecules during <i>Arabidopsis thaliana</i> Development
Lorena Aranda-Caño,
Raquel Valderrama,
Mounira Chaki,
Juan C. Begara-Morales,
Manuel Melguizo,
Juan B. Barroso
Affiliations
Lorena Aranda-Caño
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Experimental Biology, Faculty of Experimental Sciences, University Institute of Research in Olive Groves and Olive Oils, University of Jaén, E-23071 Jaén, Spain
Raquel Valderrama
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Experimental Biology, Faculty of Experimental Sciences, University Institute of Research in Olive Groves and Olive Oils, University of Jaén, E-23071 Jaén, Spain
Mounira Chaki
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Experimental Biology, Faculty of Experimental Sciences, University Institute of Research in Olive Groves and Olive Oils, University of Jaén, E-23071 Jaén, Spain
Juan C. Begara-Morales
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Experimental Biology, Faculty of Experimental Sciences, University Institute of Research in Olive Groves and Olive Oils, University of Jaén, E-23071 Jaén, Spain
Manuel Melguizo
Department of Inorganic and Organic Chemistry, Faculty of Experimental Sciences, University of Jaén, E-23071 Jaén, Spain
Juan B. Barroso
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Experimental Biology, Faculty of Experimental Sciences, University Institute of Research in Olive Groves and Olive Oils, University of Jaén, E-23071 Jaén, Spain
The non-enzymatic interaction of polyunsaturated fatty acids with nitric oxide (NO) and derived species results in the formation of nitrated fatty acids (NO2-FAs). These signaling molecules can release NO, reversibly esterify with complex lipids, and modulate protein function through the post-translational modification called nitroalkylation. To date, NO2-FAs act as signaling molecules during plant development in plant systems and are involved in defense responses against abiotic stress conditions. In this work, the previously unknown storage biomolecules of NO2-FAs in Arabidopsis thaliana were identified. In addition, the distribution of NO2-FAs in storage biomolecules during plant development was determined, with phytosterol esters (SE) and TAGs being reservoir biomolecules in seeds, which were replaced by phospholipids and proteins in the vegetative, generative, and senescence stages. The detected esterified NO2-FAs were nitro-linolenic acid (NO2-Ln), nitro-oleic acid (NO2-OA), and nitro-linoleic acid (NO2-LA). The last two were detected for the first time in Arabidopsis. The levels of the three NO2-FAs that were esterified in both lipid and protein storage biomolecules showed a decreasing pattern throughout Arabidopsis development. Esterification of NO2-FAs in phospholipids and proteins highlights their involvement in both biomembrane dynamics and signaling processes, respectively, during Arabidopsis plant development.
