Nature Communications (Apr 2018)

Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

  • Gilberto A. Santiago,
  • Jesús Vázquez,
  • Sean Courtney,
  • Katia Y. Matías,
  • Lauren E. Andersen,
  • Candimar Colón,
  • Angela E. Butler,
  • Rebecca Roulo,
  • John Bowzard,
  • Julie M. Villanueva,
  • Jorge L. Muñoz-Jordan

DOI
https://doi.org/10.1038/s41467-018-03772-1
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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The Trioplex real-time RT-PCR assay was developed for detection of Zika virus infections in areas with dengue and chikungunya transmission. Here, Santiago et al. describe the optimization and clinical performance of the assay, showing high sensitivity for detection and differentiation of the three viruses.