BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus

Ran Hu; Meichen Zhu; Si Chen; Chengxiang Li; Qianwei Zhang; Lei Gao; Xueqin Liu; Shulin Shen; Fuyou Fu; Xinfu Xu; Ying Liang; Liezhao Liu; Kun Lu; Hao Yu; Jiana Li; Cunmin Qu

Crop Journal (Apr 2023)

BnbHLH92a negatively regulates anthocyanin and proanthocyanidin biosynthesis in Brassica napus

Ran Hu,
Meichen Zhu,
Si Chen,
Chengxiang Li,
Qianwei Zhang,
Lei Gao,
Xueqin Liu,
Shulin Shen,
Fuyou Fu,
Xinfu Xu,
Ying Liang,
Liezhao Liu,
Kun Lu,
Hao Yu,
Jiana Li,
Cunmin Qu

Affiliations

Ran Hu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Meichen Zhu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Si Chen: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Chengxiang Li: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117558, Singapore
Qianwei Zhang: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Lei Gao: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Xueqin Liu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Shulin Shen: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Fuyou Fu: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 02X, Canada
Xinfu Xu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Ying Liang: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Liezhao Liu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Kun Lu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
Hao Yu: Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117558, Singapore; Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore
Jiana Li: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China; Corresponding authors.
Cunmin Qu: Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China; Corresponding authors.

Journal volume & issue: Vol. 11, no. 2
pp. 374 – 385

Abstract

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Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed, and anthocyanin and proanthocyanidins (PAs) are major seed-coat pigments. Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed. In this study, we identified a transcription factor gene BnbHLH92a (BnaA06T0441000ZS) in rapeseed. Overexpressing BnbHLH92a both in Arabidopsis and in rapeseed reduced levels of anthocyanin and PAs. Correspondingly, the expression profiles of anthocyanin and PA biosynthesis genes (TT3, BAN, TT8, TT18, and TTG1) were shown by quantitative real-time PCR to be inhibited in BnbHLH92a-overexpressing Arabidopsis seeds, indicating that BnbHLH92a represses the anthocyanin and PA biosynthesis pathway in Arabidopsis. BnbHLH92a physically interacts with the BnTTG1 protein and represses the biosynthesis of anthocyanins and PAs in rapeseed. BnbHLH92a also binds directly to the BnTT18 promoter and represses its expression. These results suggest that BnbHLH92a is a novel upstream regulator of flavonoid biosynthesis in B. napus.

Published in Crop Journal

ISSN: 2095-5421 (Print); 2214-5141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.keaipublishing.com/en/journals/the-crop-journal/

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