Internal guide RNA interactions interfere with Cas9-mediated cleavage

Summer B. Thyme; Laila Akhmetova; Tessa G. Montague; Eivind Valen; Alexander F. Schier

doi:10.1038/ncomms11750

Nature Communications (Jun 2016)

Internal guide RNA interactions interfere with Cas9-mediated cleavage

Summer B. Thyme,
Laila Akhmetova,
Tessa G. Montague,
Eivind Valen,
Alexander F. Schier

Affiliations

Summer B. Thyme: Department of Molecular and Cellular Biology, Harvard University
Laila Akhmetova: Department of Molecular and Cellular Biology, Harvard University
Tessa G. Montague: Department of Molecular and Cellular Biology, Harvard University
Eivind Valen: Department of Informatics, Computational Biology Unit, University of Bergen
Alexander F. Schier: Department of Molecular and Cellular Biology, Harvard University

DOI: https://doi.org/10.1038/ncomms11750
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 7

Abstract

Read online

While the CRISPR-Cas9 system has revolutionised molecular biology, it is still a mystery why not every guide RNA elicits target DNA cleavage. Here the authors show that genomic context and internal gRNA interactions can inhibit cleavage.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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