PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN BERBAGAI KOMBINASI ENZIM [Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes]

Abstract

The research was aimed to produce cyclodextrin from arrowroot starch using a combination of starch hydrolysis enzymes (α-amylase, α-amylase, pullulanase and glucoamylase). Cyclization process to form cyclodextrin was obtained using Cyclodextrin-glycosyl-transferase (CGTase). α-amylase showed better performance and faster to hydrolyze arrowroot starch as compared to that of β-amylase. Glucoamylase also was gave better result than pullulanase to break the α-1,6-glycosidic chain. Combination of α-amylase and glucoamylase were more efficient for hydrolysis process and cyclodextrin production.

Keywords

• arrowroot starch
• cyclodextrin
• cyclodextrin-glycosyl-transferase
• α-amylase
• α-amylase
• pullulanase
• glucoamylase