Implication for alphavirus host-cell entry and assembly indicated by a 3.5Å resolution cryo-EM structure

Lihong Chen; Ming Wang; Dongjie Zhu; Zhenzhao Sun; Jun Ma; Jinglin Wang; Lingfei Kong; Shida Wang; Zaisi Liu; Lili Wei; Yuwen He; Jingfei Wang; Xinzheng Zhang

doi:10.1038/s41467-018-07704-x

Nature Communications (Dec 2018)

Implication for alphavirus host-cell entry and assembly indicated by a 3.5Å resolution cryo-EM structure

Lihong Chen,
Ming Wang,
Dongjie Zhu,
Zhenzhao Sun,
Jun Ma,
Jinglin Wang,
Lingfei Kong,
Shida Wang,
Zaisi Liu,
Lili Wei,
Yuwen He,
Jingfei Wang,
Xinzheng Zhang

Affiliations

Lihong Chen: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Ming Wang: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Dongjie Zhu: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Zhenzhao Sun: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Jun Ma: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Jinglin Wang: Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute
Lingfei Kong: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Shida Wang: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Zaisi Liu: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Lili Wei: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Yuwen He: Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute
Jingfei Wang: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Xinzheng Zhang: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-018-07704-x
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

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Alphaviruses are enveloped RNA viruses that contain several human pathogens. Here, the authors use block-based reconstruction method and provide a 3.5 Å cryo-EM structure of sindbis virus that identifies a conserved hydrophobic pocket near the viral membrane that is stabilized by an unknown pocket factor.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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