Metabolomic approach for phytochemical assessment of Murraya koenigii fruits during different maturity stages
Manoj S. Aroor,
Vikas Dadwal,
Robin Joshi,
Mahesh Gupta
Affiliations
Manoj S. Aroor
Food and Nutraceutical Laboratory, Dietetics and Nutrition Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India
Vikas Dadwal
Food and Nutraceutical Laboratory, Dietetics and Nutrition Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India
Robin Joshi
Biotechnology Division, CSIR- Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India; Corresponding author. CSIR- Institute of Himalayan Bioresource Technology Palampur, 176061, India.
Mahesh Gupta
Food and Nutraceutical Laboratory, Dietetics and Nutrition Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India; Corresponding author. Dietetics and Nutrition Technology Division, CSIR- Institute of Himalayan Bioresource Technology Palampur, 176061, India
A detailed metabolomic study was performed on various maturation stages of Murraya koenigii fruit pulps, seed, and leaf. Among the fruit pulps, stage 6 had the highest TPC (13.27 mg/g of GAE) and TFC content (6.16 mg/g RE). The extracts also showed promising free radical scavenging activity, especially in the seed (IC50 DPPH 427 μg/mL). Metabolomics study revealed the identification of 133 metabolites in fruit pulps, seeds and leaves using the METLIN database. In silico PASS software analysis predicted the antimutagenic property of myricetin and bismurrayaquinone A. Pathway analysis revealed the phenylpropanoid biosynthesis pathway as one of the major pathways present in the fruit pulps. This detailed metabolic report of M. koenigii fruit maturation report brings a new insight into phytochemicals and their distribution in seed, pulps and leaves along with nutritive values and can be considered for nutritive and therapeutic purposes.
