浙江大学学报. 农业与生命科学版 (Jan 2015)
Phylogenetic analysis of strain CHBT-1721 with other thermophile bacteria isolated from hot springs of China
Abstract
Thermophilic bacterium, strain CHBT-1721 is the first thermophilic microorganism isolated from the hot spring of Bantang in Chaohu of Anhui Province and the phylogenetic relationship of this strain with other thermophile bacteria isolated from hot springs of China was analyzed preliminarily, which provided local government with primary data and scientific basis for utilization of Bantang hot spring water and protection of biodiversity. The strain CHBT-1721 was classified based on morphological features, physiology and biochemistry determination, and sequencing of its 16S rRNA gene. The 16S rRNA gene sequences of all the thermophilic bacteria with valid published names isolated from hot springs of China (22 thermophilic bacteria) were downloaded from the GenBank. Compared with the other 22 thermophilic bacteria, the phylogenetic diversity of strain CHBT-1721 was studied by constructing the phylogenetic tree, aligning their 16S rRNA gene sequences and analyzing their 16S rRNA secondary structures. The strain CHBT-1721 was classified to the genus of Bacillus sp. Phylogenetic analysis showed that the strain CHBT-1721 formed a cluster in the phylogenetic tree and had 94% similarity with Anoxybacillus vitaminiphilus 3nP4T in the phylogroup. Anoxybacillus vitaminiphilus 3nP4T, A. eryuanensis E-112T and A. tengchongensis T-11T (Anoxybacillus spp.) were also in the cluster.16S rRNA secondary structures comparison in the same cluster also indicated that the strain CHBT-1721 had a unique 16S rRNA secondary structure characteristic in helix 18. The strain CHBT-1721 and Anoxybacillus spp. had identical 16S rRNA gene sequences in helices 12 and 18, but not all of them had identical 16S rRNA secondary structures. This current study reveals the phylogenetic diversity of strain CHBT-1721 compared with the other 22 thermophilic bacteria. We also report that the chemical and biological parameters of the hot spring water in Bantang are similar to those in Puge of Sichuan Province.
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