IN VITRO REGENERATION OF WINTER GARLIC PLANTS (ALLIUM SATIVUM L.) FROM BULBILS

Abstract

We investigated the problem of obtaining healthy planting material of winter garlic in vitro from bulbils. The influence of the inflorescence age of winter garlic cultivars and the hormonal composition of MS basal medium on the morphogenesis of garlic bulbils in an in vitro culture is analyzed. It is found that when bulbils isolated from unopened inflorescences with a diameter of up to 25 mm are used to introduce winter garlic into an in vitro culture, one can obtain plants free from internal infections. It is proved that the cultivation of bulbils on MS medium supplemented with 2 mg/l BA and 1 mg/l NAA is accompanied by the formation of sprouts and eventually of plants with bulbils at the base. Adaptation of such plants to ex vitro conditions results in the production of single-clove bulbs, from which multi-clove bulbs of garlic is formed under open ground conditions.