Derim (Jun 2013)
Determination of resistance to Fusarium oxysporum f. sp. lycopersici via molecular markers in tomato lines
Abstract
Fusarium oxysporum f. sp. lycopersici (FOL) is common in tomato production areas where intensive production causes huge losses. Other plant species as well as biological and chemical control is insufficient to fight with the disease. The most effective solution to this problem is the use of resistant varieties. Fusarium oxysporum f. sp. lycopersici resistance has been transferred to most of the commercial varieties via classical and molecular marker-assisted selection (MAS). The use of molecular markers in the development of new varieties resistant to this disease, but not allelic race-specific resistance genes allows pyramiding to these genes at one cultivar. Markers which linked to resistance genes for FOL races (0, 1, 2) were improved and routinely used in tomato breeding programs. In this study, 450 pure tomato lines from the gene pool of tomato to the fore in terms of yield and some quality characteristics in Alata Horticultural Research Station Directorate were screened for Fusarium oxysporum f. sp. lycopersici (FOL) resistance via developed SCAR and CAPS markers linked to I-1 and I-2. The 88 tomato lines had I-1 gene and 74 of tomato lines yielded band of homozygote resistance allele for I-2. Obtained results in this study show that developed molecular markers for Fusarium oxysporum f. sp. resistance can use easily for the developing of new cultivars.
