Simultaneous determination of captopril and hydrochlorothiazide by time-resolved chemiluminescence with artificial neural network calibration

Abstract

The combined use of chemometrics and chemiluminescence (CL) measurements, with the aid of the stopped-flow mixing technique, developed a simple time-resolved CL method for the simultaneous determination of captopril (CPL) and hydrochlorothiazide (HCT). The stopped-flow technique in a continuous-flow system was employed in this work in order to emphasize the kinetic differences between the two analytes in cerium (IV)-rhodamine 6G CL system. After the flow was stopped, an initial rise of CL signal was observed for HCT standards, while a direct decay of CL signal was obtained for CPL standards. The mixed CL signal was monitored and recorded on the whole process of continuousflow/stopped-flow, and the obtained data were processed by the chemometric approach of artificial neural network. The relative prediction error (RPE) of CPL and HCT was 5.9% and 8.7%, respectively. The recoveries of CPL and HCT in tablets were found to fall in the range between 95% and 106%. The proposed method was successfully applied to the simultaneous determination of CPL and HCT in a compound pharmaceutical formulation. Keywords: time-resolved chemiluminescence, artificial neural network, captopril, hydrochlorothiazide